Microbiological studies of CО2 chamomile extract as part of a combined soft form for the treatment of wound healing

L. L. Davtyan, A. P. Shmatenko, V. A. Tarasenko, E. N. Rudenko


Baskground. In the structure of the modern pharmaceutical market, the share of drugs based on medicinal plant materials is constantly growing. A wide range of their actions is explained by the complexity of the composition of biologically active substances, the simultaneous presence of multicomponent compounds of different nature. Mild therapeutic effect, low toxicity, rare side effects, economic affordability - these are few of the advantages of therapeutic and prophylactic agents of natural origin. According to researchers, an important link is the selected method of extraction of these biologically active substances. In particular, this concerns the development of technology and the type of dosage form. Traditional technologies, which allow to obtain multicomponent mixtures of non-polar compounds of complex composition, are in most cases associated with the use of fairly “heavy” organic solvents. The aim of the study was to conduct microbiological studies of the supercritical CO2 chamomile extract from the pharmacy to develop recommendations for its possible use as part of combined soft dosage forms for treating the wound process. Materials and methods. The research materials were native CO2 extract of chamomile. Determination of the antimicrobial activity of chamomile CO2 extract was carried out by microbiological diffusion into agar on a solid nutrient medium (in accordance with the requirements of the State Pharmacopoeia of Ukraine), which is based on the ability of the antibacterial substance to inhibit the growth of microorganisms. Antimicrobial activity of the extract was studied on a nutrient medium - soy-casein agar on different test strains of microorganisms. Statistical analysis of the obtained results were made using Statistica 6.0 (StatSoft Inc., USA). The results were considered statistically reliable at P < 0,05. Results. The article presents the results of the antimicrobial activity of CO2 of Chamomile extract by the pharmaceutical method of diffusion into agar. From the presented data it follows that the test strains of Bacillus subtilis and Staphylococcus aureus are the most sensitive to the action of the indicated phytoextract and have growth inhibition zones from 10 to 15 mm. In relation to the test strains of Pseudomonas aeruginosae, Candida albicans and Saccharomyces cerevisiae, growth zones were not detected. Thus, on the basis of in vitro experimental studies, it was found that CO2 phytoextract of chamomile exhibits antimicrobial activity against the test strains of Bacillus subtilis AEC 6633 and Staphylococcus aureus ATCC 6538. Сonclusions. The complex studies conducted by the authors (literary screening, experimental work) became a logical continuation of the attention of the scientific community in many countries of the world to clean technologies. Therefore, modern researchers in the creation of new medicinal herbal remedies prefer new highly effective and environmentally friendly technologies. The presented results clearly demonstrate that supercritical CO2 chamomile extract exhibits a certain antimicrobial activity (up to 15 mm) with respect to Bacillus subtilis and Staphylococcus aureus. This confirms the possibility of using the specified phytoextract as an antimicrobial component in the composition of the combined soft dosage forms for external use, in particular for the treatment of the wound process.


wound process; drug form; microorganism; test cultures; nutrient medium; antimicrobial activity


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